Our “Z Dimension Finder with Instrument Types” tool is located at the bottom of this page.
How do you compute a cuvette’s “Z dimension”?
The spectrophotometer cuvette has a Z dimension that measures the distance from the bottom of the cuvette to solution window.
In cuvettes or flow-through cells, Z dimension indicates the small volume or narrow sample aperture.
Why is Z dimension important?
When using the ultra micro volume cuvette, sample solution must be in the optical centre so the laser beam may travel through it unobstructed. In this circumstance, data readings will likely be inaccurate or noisy. The black wall should be built to exclude light from entering the solution. Whenever the cuvette isn’t coated properly, absorbance measurements may be inaccurate.
Why does each brand (or instrument) have its own Z dimension?
Engineers at instrument businesses create instruments for maximum performance. Engineers’ light source design determines the spectrophotometer’s length, breadth, height, and flexibility. This ensures the instrument meets brochure standards.
What are some techniques to calculate my instrument’s Z dimension?
If a business equipment handbook is going to be complete it must include the Z dimension, also known as the height of the centre strut or beam.
What is the Z dimension of my instrument?
Depending on where the light source has located, the Z dimension will vary from instrument to instrument. Learning how to compute the Z dimension on your own is a valuable skill.ethod 1 Z dimension check
Checking your instrument’s Z dimension is simple. Steps:
1. Prepare a 12-by-100-millimeter piece of white cardstock or thick paper.
2. Pierce the paper using a ballpoint pen. It should be 3mm in diameter and 8.5mm from the card’s base. The card’s opposite end has a 15-mm hole.
3. Turn on your spectrophotometer, choose % T mode, and pick a wavelength.
4.Use the prepared card to turn the cell holder into a cuvette. Using either the 8.5 mm or 15 mm end, which both include a light-passing hole, you may establish your instrument’s Z dimension.
5.If none of these possibilities work, construct a third card with different-sized holes (at least 20 mm in diameter). Check out our table “Z-dimension Finder using Instrument Models” for ideas.
Measure Z dimension It’s simpler to measure the Z dimension if the sample container is easily accessible.
1. Making the instrument visible by changing its wavelength (e.g. 546 nm).
2. Put white card in sample slot.
3. Using the laser beam, mark the card where it is.
Z dimension is near to card mark-to-base distance.